Non-specific degradation of transcripts promotes plasmid clearance during type III-A CRISPR–Cas immunity
نویسندگان
چکیده
منابع مشابه
Degradation of Phage Transcripts by CRISPR-Associated RNases Enables Type III CRISPR-Cas Immunity
Type III-A CRISPR-Cas systems defend prokaryotes against viral infection using CRISPR RNA (crRNA)-guided nucleases that perform co-transcriptional cleavage of the viral target DNA and its transcripts. Whereas DNA cleavage is essential for immunity, the function of RNA targeting is unknown. Here, we show that transcription-dependent targeting results in a sharp increase of viral genomes in the h...
متن کاملA Type III protein-RNA toxin-antitoxin system from Bacillus thuringiensis promotes plasmid retention during spore development
Members of the Bacillus cereus sensu lato group of bacteria often contain multiple large plasmids, including those encoding virulence factors in B. anthracis. Bacillus species can develop into spores in response to stress. During sporulation the genomic content of the cell is heavily compressed, which could result in counterselection of extrachromosomal genomic elements, unless they have robust...
متن کاملUntemplated oligoadenylation promotes degradation of RISC-cleaved transcripts.
Double-stranded RNA, processed to small interfering RNAs (siRNAs) by Dicer and incorporated into the RNA-induced silencing complex (RISC), triggers gene silencing by a variety of pathways in eukaryotes. RNA interference involving the degradation of homologous transcripts is the best-characterized mechanism. However, the fate of the RNA fragments resulting from siRNA-directed cleavage is poorly ...
متن کاملStudies on Natural Immunity to Pneumococcus Type Iii Iv. Observations on a Non-type Specific Humoral Factor Involved in Resistance to Pneumococcus Type Iii By
Experimental evidence presented in Paper III* indicates that removal and destruction of both rabbit virulent and avirulent strains of Pneumococcus Type III in the normal animal are brought about by the same means, namely, the phagocytic cells of the body, provided the integrity of the capsule becomes impaired. It leaves unanswered, however, the interesting q~aestion as to whether this phagocyto...
متن کاملذخیره در منابع من
با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید
ژورنال
عنوان ژورنال: Nature Microbiology
سال: 2019
ISSN: 2058-5276
DOI: 10.1038/s41564-018-0353-x